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Chris Winter, a sleep neurologist, published a study in which he concluded that sleep deprivation causes the skin of the under-eye area to appear dull, and the dilated blood vessels make the skin appear darker in complexion

Western blot (WB) analysis For WBs, cells were extracted in RIPA buffer (Sigma-Aldrich, USA), separated by 10% SDS-PAGE, and transferred onto a polyvinylidene difluoride membrane (Millipore, USA)

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moreover, by upregulating VEGF, hypoxia inhibits spermatogenesis and decreases sperm motility and count 49

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Further research by Lingsi Zeng et al