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PMID 19893032

Replacement of Asn450 by glutamine (N450Q), aspartate (N450D), and alanine (N450A) showed a significant increase in transpeptidase activity up to 3.6-fold enhancement and minor maturational defects, suggesting significant contribution of this residue in substrate binding and catalysis

Nakayasu ES, Gritsenko M, Piehowski PD et al (2021) Tutorial: best practices and considerations for mass-spectrometry-based protein biomarker discovery and validation

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Since that time, there have been a lot of investigations with findings reported in hundreds of articles published on various reputable resources, including the PubMed database, maintained by the U.S