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We quantified the level of test compounds or control compounds in the samples using the software of MassLynx (v4.2) to set up a gradient of 23100% B (A: water + 0.2% formic acid, B: 4:1 methanol/isopropanol) in ESI+ mode on a LC-MS equipped with a Waters H-Class Plus UPLC (Acquity UPLC BEH C18, 2.1 50 mm, 1.7 m) connected to a Xevo G2-XS QTOF mass spectrometer

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Hazen, who also holds the Jan Bleeksma Chair in Vascular Cell Biology and Atherosclerosis, has previously linked TMAO to an increased risk of cardiovascular disease and has shown it can be a powerful tool for predicting future heart attacks, stroke and death in multiple patient populations

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