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Nucleic Acids Res 48 , 1243612452 (2020)

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AtSLIM1 transcript levels were significantly higher in the 35S::SLIM1 lines than in Col-0, EV (empty vector control), and slim1-cr ( Figure 1A )

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(11)Fmoc-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- (11) Preparation of Fmoc-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin: (10)Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-1050530105010603DMF3DMF2DMFFmoc-Pro-OHDIEATBTU/HOBtHBTU/HOBtBOP/HOBt105010603DMF3DMF2 Fmoc-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- In the Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin of step (10), add decapping reagent 1050 and react for 5 30 minutes, dry with a vacuum pump, re-add the decapping reagent at 10-50 C for 10-60 minutes, dry, wash with isopropanol for 3 times, DMF for 3 times, re-distilled DMF for 2 times, add DMF Dissolved Fmoc-Pro-OH, DIEA, TBTU/HOBt or HBTU/HOBt or BOP/HOBt mixture, reacted at 10-50C for 10-60 minutes, dried with a vacuum pump, washed 3 times with isopropanol and 3 times with DMF times, the DMF that has been steamed again was washed 2 times, and was drained to obtain Fmoc-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin

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