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With bacteriostatic water, those bacteria cannot establish a colony

Excess 5-(Iodoacetamido)fluorescein was removed using a PD MidiTrap G-25 column followed by size-exclusion chromatography (Superdex 75) in 25 mM HEPES and 150 mM NaCl to yield fluorescently labelled UB and UB K0 (UB* and UB K0*, respectively)

smegmatis and testing efficiency of ESX secretion before and after gene knockout, specific genes can be identified as necessary for ESX secretion

Discuss discrepant results with your local laboratory

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Among them, DT is an anthracene compound derived by substituting hydrogen atoms at C-1 and C-8 with OH groups and adding an oxygen substituent at C-9