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Kim MJ, Hyun MK, Kim D et al (2004) Dynamic kinetic resolution of secondary alcohols by enzyme-metal combinations in ionic liquid

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The clarified supernatant was loaded onto Ni-NTA resin (Nacalai Tesque) pre-equilibrated with 50 mM Tris-HCl (pH 8.0), 8 M urea, and 20 mM imidazole