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62 , e202217716 (2023)

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References: Wu, G., Fang, Y

In brief, the reaction was initiated at 40C upon the addition of 10 l CFX into the reaction solution (1 ml) consisting of 0.1 M TrisHCl buffer (pH 8.0), 6 mM D,L-hydroxyphenyl hydantoin (HPH), and 0.5 mM MnCl 2

El pH de la fase mvil puede ser alterado para favorecer la adsorcin o elucin de las protenas