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Indirect staining for the HIS-tag was performed using anti-HIS (Abcam catalog number S2917, RRID: AB_10641949) in combination with anti-mouse-HRP (R and D Systems catalog number MAB050H, RRID: AB_357354)

Synthetic lethality An effect in which two inhibitors or losses of function that, individually, do not produce death in cancer cells, if combined, synergistically induce death

Enzymes involved in the SGOC pathway are linked to tumor growth, metastasis, and resistance to therapies

This phenomenon disrupts the balance between bone formation and resorption, thereby promoting the production of proinflammatory cytokines, which can interfere with the osteoprotegerin/receptor activator of NFB ligand balance, creating a pro-osteoclastogenesis environment favouring bone loss [48]

Figure 2 Disruption of Methyl Donor Balance in NNMT Overexpression Cancer Cells NNMT is also a key node in methyl donor metabolism

doi: 10.3390/biomedicines9101356