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After fixation of the biopsy specimens, free floating 50-m-thick sections were cut and stained by using rabbit anti-protein gene product 9.5 antibody (Dako Netherlands BV, Eindhoven, the Netherlands) and visualized using a goat anti-rabbit Alexa fluor 488 antibody (Invitrogen,/Life Technologies, Carlsbad, CA, USA)

Wellington, New Zealand, 1998

If cloudiness persists, let the vial rest for 5-10 minutes and swirl again

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