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The diaPASEF raw file processing and controlling peptide and protein level false discovery rates, assembling proteins from peptides, and protein quantification from peptides was performed using library free analysis in DIA-NN 1.8 155 searched against a Swissprot human database (January 2021)

pJL2, Pmrp-1 SphI fusion F 5actgGCATGCccagtcaacgtttcgataagttgg, mrp-1 KpnI fusion R 5actgGGTACCcccacaacatttgcatcttttgccattgaatag

Indeed, like a domino effect, the shutting down process happens in steps

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doi: 10.1186/1678-9199-20-13 8 BarbosaANFerreiraRSde CarvalhoFCTSchuelter-TrevisolFMendesMBMendonaBCet al

It is a tightly regulated reaction that depends on both the availability of the amino acids (cysteine, glycine, and glutamate) and the rate-limiting enzyme, glutamate-cysteine ligase ( GCL )