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doi: 10.1016/j.scitotenv.2013.03.083 198 ZakyA

Isolation of WAT stromal vascular cells and adipocyte differentiation To isolate the stromal vascular fraction, minced iWAT (combined depots from 45 mice per genotype) from 46-week-old mice was digested in 1 mg ml 1 collagenase D (Roche) with 1.5% BSA in sterile Hanks Balanced Salt Solution and incubated in a 37 C shaking water bath for 1 h

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[DOI] [PMC free article] [PubMed] [Google Scholar] 61.Cognitive-behavioral therapy improves weight loss and quality of life in women with polycystic ovary syndrome: a pilot randomized clinical trial

It downregulates the expression of pro-oxidant enzymes, such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), which are notorious for driving systemic inflammation