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Once all parameters derived from the thermodynamic cycle shown (Figure 6B) as well as the impact of the cellular buffer and any change in the number of sensor molecules per cell (in high and in low metal) for each metallosensor are considered, response curves centered on K d S that relate fractional transcription output to the intracellular availability of the cognate metal are obtained (Figure 8A)

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Gently swirl the vial (do not shake) until the powder is fully dissolved and the solution is clear and colorless

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Keep out of direct light