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This conclusion is further supported by the observation that mice lacking Epac1 , which encodes one of the two isoforms of EPAC in mammals, are more resistant to DIO and have improved glucose tolerance and heightened central leptin sensitivity, as measured through STAT3 activation, compared with control mice 98

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Youll typically reconstitute it using sterile bacteriostatic water, though sterile saline works for acute systemic preparations

Each sample was analyzed with three biological replicates and three technical duplications