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Cells at rest have a GSH/GSSG ratio exceeding 100, while in those exposed to oxidant stress the ratio drops as low as between 10 and 1, making this ratio a reasonable predictor of the cellular redox state
DMSO is diluted on exposure to air

10.1074/jbc.M115.659680 [DOI] [PMC free article] [PubMed] [Google Scholar] Toyokuni S., Okamoto K., Yodoi J., and Hiai H

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The mean background value for each channel was further used in automated image analysis

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