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Diabetes TrialNet the Immune Tolerance Network Rapamycin/IL-2 combination therapy in patients with type 1 diabetes augments Tregs yet transiently impairs beta-cell function Diabetes 61 9: 2340-8

[DOI] [PMC free article] [PubMed] [Google Scholar] 57.Golomb BA, Evans MA, Dimsdale JE, White HL

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For the extraction, methanol and internal standards were added, followed by incubation for 15 min at 30C on a thermal shaker (650 rpm, TS1 ThermoShaker, Biometra, Gttingen, Germany), centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany), evaporation, and centrifugation with methanol again