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The different nature of these residues is suggested to be responsible for the differences in catalytic properties of mesophilic and extremophilic GGTs ( FIGURE 5 Recent crystallographic studies on L-Glu-bound BlGGT demonstrated that its active site channel constitutes two extensive pockets at the intersubunit interface lined mainly by hydrophobic residues

Compared with control animals, mice receiving L-carnitine exhibited a significant reduction in adipose tissue weight, better glucose tolerance, and lower plasma triglyceride and cholesterol levels

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Archin NM, Espeseth A, Parker D, Cheema M, Hazuda D, Margolis DM

The reaction was incubated at 37 C, and after 30 min the enzyme activity was stopped by the addition of 4.5 l DNase Inactivation Reagent, followed by vortexing, and incubation for 5 min at room temperature

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