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A series of studies have shown association linking cigarette smoking and nicotine toxicity to glutathione depletion within periodontal tissues.[4] Studies of betel nut chewing, and periodontitis have shown that arcea nut alkaloid, arecoline-induced thiol depletion in periodontal ligament fibroblasts may render them more susceptible to the effects of nicotine;[57,58] glutathione protection negated nicotine toxicity;[41] and that cigarette smoke decreased periodontal ligament fibroblast glutathione levels in a dose-dependent manner and stimulated stress-specific genes.[44] As already discussed, GST is xenobiotic metabolizing enzyme required for the detoxification reactions and utilizes free glutathione

Its generally best to avoid heavy meals or large amounts of caffeine before your infusion

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