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Human ileal and colonic tissue was obtained from patients undergoing bowel resection for cancer or stoma reversal

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Transfections and cell culture Human and pGLP-1R cDNAs were isogenically integrated into FlpIn-Chinese hamster ovary (FlpInCHO) cells (Invitrogen) and selection of receptor-expressing cells accomplished by treatment with 600 g/ml hygromycin B as described previously 46