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Adapted from Holt et al
Renal cortical extracts previously quantified were adjusted to 0.5 g/L, and then 2 L of each diluted extract was incubated with 73 L test solution (0.4 M boric acid, 0.9 M NaCl pH 8.3) with the substrate N-hippuryl-L-histidyl-L-leucine for 25 min at 37C
