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Primers are: ER36- forward: 5-GCGAATTCACCATGGCTATGGAATCTGCCAAG-3 ER36- Reverse: 5-GCGGGATCCCTGTGATCTTGTCCAGGACTC-3 All plasmids were verified by restriction enzyme digestion and DNA sequencing

Leg cramps, commonly referred to as muscle spasms or Charley horses , are abrupt and often agonizing occurrences that catch individuals off guard, leaving them in search of immediate relief

However, it causes substantially more injection site reactions

1a and b)

4mg weekly: Some trial participants started at 4mg

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