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The bulky A350 6.39b W and K351 6.40b A mutants almost abolished the maximal response (Emax) of GLP-1R-mediated cAMP accumulation in presence of compound 2, while V332 5.62b A, K346 6.35b A and L349 6.38b A mutants greatly elevated basal cAMP activities but significantly diminished the efficacy of the response upon stimulation by either compound 2 or GLP-1, suggesting these mutations affect the kink of TM6 required for receptor activation

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At steady state, the difference between injecting on day six versus day seven is smaller in absolute terms than during the initial titration phase

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