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doi: 10.1001/jamanetworkopen.2024.57232

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A lyophilized aliquot of the peptide was taken in deionized water ( ~ 2 mg/mL peptide concentration) and a clear solution was obtained by the addition of acetonitrile, ammonium hydroxide (up to 1%), or acetic acid (up to 9%) followed by sonication

The gap in the logic is the same one as with methionine: correcting a deficiency is not the same as producing weight loss in someone who is replete

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