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After 1-hour incubation in blocking buffer (2% BSA in PBS containing 0.1% Tween 20), coverslips were incubated with primary antibodies against glucagon (R&D Systems, catalog MAB1249, 1:100) and EphA4 (ABclonal, catalog A8346, 1:100

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At the pre-onset stage, PID 11, NLY01 reduces the percentage of activated myeloid cells (CD11b + MHCII + ), mature dendritic cells (CD11c + MHCII + ), and effector T cells (CD4 + CD44 + ) in the spleen [121]

A shave biopsy of a lesion was then taken, and the patient was started on a prednisone taper