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lag-1(oz536) (rollers, with SEC in) was verified through Sanger sequencing, followed by two times outcrosses with wild type prior to heat shock to remove the SEC [68] to generate lag-1(oz536oz537) lag-1(ozIs43[lag-1 :: 3xFLAG :: BioTag + unc-119]) fosmid transgenic line ( S5A Fig ) was generated through insertion of the 3xFLAG and BioTag encoded peptide sequence at the C-termimus of LAG-1 in pCC1FOS vector (Source BioScience, CBGtg9050C1288D) by Recombinering [70]