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Additionally, ROS levels assessed 48 h post-exposure to CS using the DCFDA/H2DCFDA assay displayed a significant increase in the CS-exposed cells compared to CTRL samples (Fig

Cells were washed with cold PBS and lysed in a buffer containing 10 mmol/L Tris-HCl (pH 7.4), 50 mmol/L NaCl, 5 mmol/L ethylene diamine tetra-acetic acid (EDTA), 1% Nonidet P-40
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