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2.7 ELISA CP-containing lysates (and non-template controls) were diluted 1:200, 1:500 and 1:1000 in coating buffer (15 mM Na 2 CO 3 , 35 mM NaHCO 3 , pH 9.6) and 100-L aliquots were coated onto high-binding microtiter plates (Greiner Bio-one) for 4 h at 37 C

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References [edit] "FDA to begin regulating electronic cigarettes"

T-PCR targeting the coat protein gene offers high sensitivity and serotype differentiation via sequencing or restriction analysis

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