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Five to ten micro liters of the filtered smoke solution was diluted with mobile phase and 20 l of this diluted solution was injected to the HPLC column with the UV detector set at 294 nm

1920s-1940s: 10-20 cigarettes common 1950s-1960s: 20 cigarettes became standard 1970s-1980s: 20 cigarettes universal standard Collectible packs Often contain fewer or no cigarettes

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The move "proved effective," and the company already has contacted the city "to talk about finding a solution," mayoral spokeswoman Catherine Turco said