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For detecting ROS by IF, slides were incubated with dihydroethidium (DHE) solution (Sigma, D7008) at 37 C for 30 min, succeeded by counterstaining with DAPI (Beyotime, C1002) for 10 min at room temperature in the dark
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Some of the common mistakes include: Using F10.239 for patients with seizures This should be F10.232 instead
Lightly cover them by raking them gently with your hand
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Black lettering on yellow ground, en verso
