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Tar affects smoke density and flavor intensitylower tar results in lighter smoke that feels less heavy on the lungs
After incubation, cells were rewashed with PBS and imaged under a fluorescence microscope (Axiovert 40CFL with Axiocam MRm, Zeiss, Germany)

10 , 11 The epithelial reaction to antigen exposure begins with the secretion of IL33, TSLP, and IL25, activating innate type 2 lymphoid cells (ILC2) in mucosa and the production of atopic T helper 2 lymphocyte profile cytokines (Th2), with the consequent progression of allergic inflammatory cascade due to its effects on mast cells, macrophages, eosinophils, and CD4 + T lymphocytes
Nicotine is actually toxic to humans in high doses much higher than those obtained from smoking cigarettes

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