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Cloning and quantitative real-time PCR (qRT-PCR) analysis of NtHQT genes The coding sequences of NtHQT1-4 were cloned by PCR amplification from cDNA isolated from tobacco leaves and roots with gene-specific primers (Supplementary Table S1)

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Several additives are known to have toxic properties

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Like any smokable product, informed use and realistic expectations matter

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Remember, tobacco doesnt need to be cold, it needs to be protected

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