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The second study demonstrated that exposure of human bronchial epithelial cells (BEAS-2B) over a 12-week period to total particulate matter produced from the THS2.2 heated tobacco product did not produce an increase in inflammatory mediators at a five-fold higher concentration when compared to total particulate matter from the 3R4F Kentucky reference cigarette (van der Toorn et al., 2018)

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Smoking increased the expression of tissue inhibitor of metalloproteinase-1 and procollagen-alpha2 (I) peptide in obese Zucker rats who were exposed to 2 cigarettes/day, 5 days per week, for 4 weeks, but not in the control rats who were not exposed to cigarette smoke

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