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Moreover, further attenuation strategies based on malleability of the rhabdovirus genome, strategies such as decreasing the expression level of viral genes by manipulating their position down the expression gradient (Flanagan et al., 2003) or by G gene truncations and N gene translocations (Figure 6E), or inserting the foreign gene upstream the N gene while relocating the N gene downstream the M gene and truncating VSV G protein (Figure 6F) were also adapted to rescue a recombinant VSV based HIV-1 vaccine ( The inherent genomic characteristics of rhabdoviruses, such as having well-defined transcription start/stop signals (Stillman and Whitt, 1997), and the ability to accommodate large inserts while retaining high level expression rates (Haglund et al., 2000), together with negligible seropositivity in the human population (Roberts et al., 1999) made VSV a lucrative candidate for vaccine development and as vector for a number of biomedical applications such as recombinant viral vaccines (Safronetz et al., 2015

In 2019 alone, there were a staggering 8.7 million deaths worldwide directly attributable to tobacco consumption 2

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Distribution involves secure transport channels due to regulatory controls and taxation policies

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