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To me, it is just very opportunistic what is happening"

After the 48 h, the supernatant was removed and a 25 mM of culture medium containing 0.5 mg/mL of MTT salt solution was added to each well and it was again adjusted in a 5% carbon dioxide incubator at 37 C for 4 h

Control samples were also prepared by the same method

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Given the pervasive and multifaceted detrimental effects of tobacco on liver health, promoting and facilitating smoking cessation should be a cornerstone of clinical practice for all healthcare professionals, particularly hepatologists and gastroenterologists