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Cell lysates were analyzed for luciferase activity using the Dual-Luciferase Reporter Assay System (Promega)

We also provided preliminary evidence suggesting that, as in the BBDP rat model of the disease, zonulin up-regulation precedes the onset of diabetes in T1D patients (11)

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however, excessive autophagy may synergistically enhance cuproptosis ( In immunotherapy, low PD-L1 expression in tumor cells is often associated with a poor immune response (Zhang et al., 2023)

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Furthermore, tissue-specific transcriptional signatures in V1V0 indicated significant activation of tissue damage markers associated with esophagus mucosa and heart (Fig