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For each rate study, 50 M of reduced protein was incubated in a reaction mixture containing 1 mM DCP-Bio1, 25 mM potassium phosphate (pH 7.0), 100 M DTPA (an aggressive chelating reagent) with one (AhpC) or two (papain) equivalents of H 2 O 2 (fRMsr was oxidized with a 100-fold excess of methionine sulfoxide)

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