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Then, the mixture was incubated for 1 h before centrifugation at 15000 g for 15 min at 4 C

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doi: 10.2174/1389557514666140219103138

Importantly, and pointing toward oxidative stress as a mechanism implicated in hyperglycemia-induced glycocalyx degradation, the reduction in glycocalyx volume was largely prevented by treatment with an antioxidant agent (214)