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After removing primary antibody and washing three times with PBS, iHLOs were incubated with secondary antibody diluted in antibody dilution buffer for 2 h at room temperature

investigated several aromatic carbonyl compounds, including 2,4-DHBA, DHAP, 2,3,5-trihydroxybenzaldehyde, and 2,4-dinitrobenzaldehyde, for use as potential reactive matrices for primary amines and used MALDI-MS to analyze monoamines, diamines, and polyamines

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Since serine and glycine are considered nonessential, their depletion can be tolerated in vivo