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Understanding how the microbiome is involved in establishing, maintaining, and reversing obesity has been an active area of research 14,15,16 , and has included studies investigating links between the microbiome and obesity-related comorbidities such as non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) 17,18

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For the recordings, slices were transferred to a recording chamber continuously perfused with aCSF and visualized on an upright microscope (Zeiss, Germany) using a 60x water immersion objective, infrared differential interference contrast optics, and a CMOS camera (Hamamatsu, Japan)