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The supernatant was then centrifuged at 12,000 g for 15 minutes at 4 C, resulting in the supernatant as the cytoplasm fraction and the pellet containing the mitochondrial fraction

doi: 10.1073/pnas.171320998

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These compounds not only help beneficial microbes survive but also support the production of gut-derived metabolites important for brain and gut health (Kang and Zivkovic, 2021)

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Deficiency in B12 can lead to demyelination, resulting in neurological symptoms such as numbness, tingling, impaired coordination, and cognitive decline