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For transwell assays, A-431 cells seeded in 24-well plates were covered with 750 L fresh RPMI-1640 medium with or without 10 mg/mL albumin
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The key lies in maintaining optimal, not excessive, intake levels

For the best possible outcome it is advised that you initially have loading doses - this would be carried out as 1 injection every 3-4 weeks

Jastreboff AM, Aronne LJ, Ahmad NN, et al
