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Description
Briefly, BALF was sonicated (Sonics), centrifuged for 15 min at 14,000 g at 4 C and filtered with pre-rinsed (5% methanol and water) syringe (Monoject, Covidien) and 0.22um PES filter to remove remaining particulates
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Han, F., Li, S., Yang, Y
They studied methylation of the p53, p16 , Hypermethylated in cancer 1 protein ( HIC1) , and IL-6 genes as well as of Alu and LINE-1 repetitive elements
This makes supplementation essential for many people
