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Serial dilution spot assays and growth curves In a typical growth experiment, single colonies of the WT, gsh2 mutants, or gsh2::GSH2 strains were selected from an agar plate and incubated at 30C in YPD medium overnight with shaking at 220 rpm

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The FLAG-tag is underlined, and the stop codon is indicated with an asterisk

In addition, iCAFs inhibited several hallmark pathways, such as the TGF-beta signaling, WNT signaling, Hippo signaling, and mTOR signaling pathways, suggesting the transition of iCAFs to an antitumor phenotype (Figure S10F)

Carnitine, on the other hand, is essential for mitochondrial fatty acid transport and -oxidation, may enhance lipid metabolism, and mitigates hepatic steatosis [97,98,99]