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SDS-PAGE was performed at 60 volt (V) for 3 h 30 min to separate proteins, followed by transferred to polyvinylidene fluoride (PVDF) membranes (Merck, USA) using a semi-dry transfer system (Trans-Blot SD, Bio-Rad, USA) at 20 V and 200 milliampere (mA) for 45 min

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3-PBLG was prepared in TFA- d 1 for proton nuclear magnetic resonance ( 1 H NMR) measurement on an AVANCE III HD 600 NMR

Both drugs are forms of amphetamines, but the difference is Adderall includes two amphetamine salts called amphetamine sulfate and dextroamphetamine sulfate