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Cyanocobalamin, a slightly toxic, synthetic form, is especially dangerous to pernicious anemia patients

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Mass spectrometry We performed similar in vitro kinase assays with excess non-radioactive ATP and measured the phosphorylation of recombinant GST-tagged WT ArfGAP1 by GST-N-LRRK2 (WT, G2019S and D1994A) using mass spectrometry

The underlying logic is straightforward: if each component supports a different aspect of fat metabolism, combining all three could provide synergistic metabolic support