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After the exposure to the acute stressor, salivary cortisol levels showed a fast increase within 25 min after the stressor, and reached maximum levels after 160 min (13:00 h: 3.56 1.09 pg/l) which is comparable to urinary cortisol levels

route caused brain tissue alterations, including oedema, neuron death via necrosis, and hyperplasia [113,114]

This approach enables timely treatment whilst reducing the need for frequent healthcare appointments

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This technique is widely used in biochemistry, molecular biology, and pharmacology to characterize proteins, metabolites, and other chemical compounds [74]

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