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After enzyme extraction, protein concentration was assessed according to the method of Kruger (1994)
Finally, the slides were fixed with Antifade Mounting Medium (S2100, Solarbio), and images were acquired using a confocal laser scanning microscope (LSM900, ZEISS)
10.3233/jad-190474 J
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Kwatia MA, Doyle CB, Cho W, Enhorning G, Ackerman SJ
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