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Immunocytochemistry of 3D neurospheres and confocal imaging Three-week old neurospheres were fixed with 4% formaldehyde for 25 min, washed twice with PBS 1X (w/o calcium and magnesium) for 7 min, and stored in PBS 1X at 4 C prior to staining

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Acknowledgments This work was supported by the University of Zagreb, Zagreb, Croatia (grant BM 099) and the Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek

doi: 10.1016/j.etap.2023.104324 601 TruchetD