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For RNA extraction, tissues were homogenized using a bead homogenizer in TRIzol Reagent, catalog # 15596026 (ThermoFisher Scientific), and RNA was extracted according to manufacturers protocols and purified using Econospin RNA Mini Spin Columns for RNA (Ephoch Life Sciences, Missouri City, TX, catalog #1940-250) following manufacturers protocols

Patients are randomly assigned to treatment or placebo groups, and neither patients nor evaluators know who received which (double-blind)

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It simply means that in the GHK-Cu vs BPC-157 comparison for deeper structural tissue research, BPC-157 is usually the more common choice

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