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Diabetes 50:14641471 Lodge JK, Youn H, Handelman G (1997) Natural sources of lipoic acid: determination of lipoyllysine released from protease-digested tissues by high performance liquid chromatography incorporating electrochemical detection

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(Lond.) 126 , 207221, doi: 10.1042/CS20130117 (2014)

Then 20 l serum was mixed with 2 l 200 mM NEM (Thermo Scientific, 040526.06) and extracted with 80% methanol

Optic Nerve Analysis A 3-mm section of optic nerve proximal to the globe was isolated, post-fixed for 1 hour in 4% PFA, and prepared for embedding and semi-thin sectioning, as previously described [39]