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Description
Feb 8 1988;228(1):179-181

Demonstration that polyol accumulation is responsible for diabetic cataract by the use of transgenic mice expressing the aldose reductase gene in the lens

The binding of the cobalamin-IF complex is selective and rapid and requires Ca 2+ , but it is not energy dependent

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Membranes were washed 3x for 10 min with TBST and subsequently incubated for 1 h at room temperature with appropriate horseradish peroxidase-conjugated secondary antibodies (Sigma-Aldrich, NA934-1ML or NA931-1ML) diluted (1:10,000) in 5% (w/v) milk TBST

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